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who.int/ipcs/food/jmpr/arfd/en/index.html (accessed 2 . Pasteurization was mostly due to thermal effects About 50 plants were still using this technology until the 1950s, but they were shut down as a result of competition from other methods of heating and increasing costs of electricity. The use of intensive electric eld pulses on inactivation of microorganisms in foods was patented by Maxwell laboratories in the mid-1980s (2, 5). Pilot scale PEF equipment is available in processing apple juice, cider, and orange juice to produce foods with extended shelf life with fresh-like quality. Electroporation is thought to be the major mechanism causing microbial inactivation, which implies the phenomenon that pores are created in cell membranes when the cell is placed in an electric eld with a short pulse (1 100 s), causing cells to swell and rupture (1). A natural pressure gradient exists across cellular membranes, so that when a cell is placed in an electric eld, the transmembrane potential increases. If the applied electrical eld is more than a certain critical value, then the cell wall ruptures. While the complete mechanism of cell wall breakdown in an electric eld is not clearly understood, this observed phenomenon is used regularly in creating pores in a cell membrane. The technique has been used in the eld of biotechnology to introduce foreign DNA into a cell. A eld strength above 15 kV/cm is required for destruction of microbial membranes (6). In addition, reactive free radicals or electrolysis products are generated during PEF process that may also contribute to the microbial inactivation. for ASP.NET Control to generate, create barcode image in .Related: EAN-8 Printing .NET , Make UPC-E .NET , Creating ISBN .NET

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